Rapid identification of acid fast bacilli in clinical specimens is of utmost importance to make therapeutic decision and to minimize the risk of further epidemic spread of the disease. Acid fast smear although a tool for rapid diagnosis of AFB, is not very helpful for the presumptive diagnosis of tuberculosis. Body fluids samples from eighty patients with suspected tuberculosis were included in this study. They were processed according to standard laboratory procedure and concentrated by cytosieve technique. Culture and cytosieve technique results were correlated. Of the eighty clinical specimens there were only three (2.8%) specimens which shows culture negativity and cytosieve technique positivity. All the other specimens shows good correlation. Overall, there were 10 culture positive specimens and 13 cytosieve techniques positive specimens. The sensitivity, efficiency, and rapidity of cytosieve technique resulted in increased detection of mycobacteria in clinical specimen. In addition, the analyst needs to screen only a small field. The simplicity of this method will enable qualified technologist to rapidly diagnose body fluids for AFB positivity in field site at clinics and small laboratories.
The study seeks to compare the cytosieve membrane filtration against centrifugation as two concentration techniques in the diagnosis of acid-fast bacilli (AFB) in the body fluids and effusions. It also aims to determine the sensitivity of the cytosieve technique in detecting AFB and compare it to the routine AFB staining of slide smears.