Filariasis is a disease which leads to permanent and long-term disability among afflicted individuals. Elephantiasis is one of its manifestations. Since Bancroftian filariasis has a nocturnal periodicity, diagnosis is confirmed by finding the microfilaria in the peripheral blood smear taken during the night. The research aimed to find the validity of daytime peripheral blood smear collection as an alternative diagnostic tool in the diagnosis of Filariasis. The study area was Himokilan Island. Hindang, leyte. In 2000, the prevalence of Filariasis in said area was 6.73%. Three hundred ten (310) respondents were enrolled in the study using the inclusion and exclusion criteria. Blood was extracted from each respondent twice, once during the day another during the night using thin and thick smears. Based on the study, the prevalence of Filariasis using the Daytime Peripheral Blood Smear (DPBS) was 0.32% while using the Nightime Peripheral Blood Smear (NPBS) was 1.0%. The sensitivity of DPBS using the NPBS as gold standard was 33.3% while the specificity was 100%. Since the specificity of DPBS is high, it can be used n the follow-up of patients under treatment. However, since the sensitivity is quite low and unstable due to the small sample size, we cannot conclude that it can be used as an alternative diagnostic tool in the screening of patients suspected of having Filariasis.