Report an error   


Submitted: 21 March 2017 Modified: 05 June 2018
HERDIN Record #: R07-CIM-17032115021763

A comparative study between the graded results of Sputum AFB Smears prepared from the standardized approach and non-standardized approach in smear preparation.

Joni B. Abdon,
Alfred Adams  M. Angulo,
Erwin Cempron,
Ives Brylloe Villas,
Faith Caren Castro,
Anjorie Pearl Co,
Kathleen Joyce C. Del Carmen,
Monica Janine C. Lee,
Francine B. Lucero,
Venees Sherry P. Narvaez,
Danica Julia P. Oliveros,
Jan Angeli T. Ong,
Karen L. Opay,
Kathleen B. Rabe,
Maria Rheena Flor  C. Zamora,
Eliel Paglinawan

See More

Sputum smear microscopy is widely used in the diagnosis or microscopists may differ in their  approach to the procedure such that the DOH-NTP established Standard Guidelines to encourage uniformity and reproducibility of the results.This study aims to determine whether or not Non-Standard smear preparation,that is not complaint to the standard guidelines set by DOG-NTP differ from that using the Standard in terms of the degree of agreement of their graded results.Cohen's kappa statistics was used to determine such parameter which yielded  a value that implies only fair agreement (κ=0.359) among the two approaches. The two smearing methods were then subjected to quality control (QC) which affirmed that the Standard set-up did comply with the standard guidelines in terms of quality while the Non-Standard complied less. However, it appears that the Non-Standard set-up has much greater agreement with the QC's graded results (κ=0.366) compared to the Standard set up (Κ=0.198), albeit still considered a fair, and poor agreement respectively. With this, we recommend further studies considering this apparent paradox that may be due to reader error. Nonetheless, given that there is considerate disagreement between the two approaches, this study reinforces the idea that sputum smear microscopy must adhere to Standard Guidelines to ensure the quality of prepared slides.

Publication Type
Thesis/Dissertations
Thesis Degree
MD
Specialization
Medicine
Publication Date
April 2015
LocationLocation CodeAvailable FormatAvailability
Cebu Institute of Medicine Abstract Print Format
Cebu Institute of Medicine Abstract Print Format
1. Glaziou, P. , Floyd, K. , Raviglione, M. . "Global Burden and Epidemiology of Tuberculosis" Clinics in Chest Medicine 30(4): 621-636, 2009
2. Glaziou, P. , Floyd, K. , Raviglione, M. . "Global Burden and Epidemiology of Tuberculosis" Clinics in Chest Medicine 30(4): 621-636, 2009
3. Steingart , K. . "Sputum Processing Methods to Improve the Sensitivity of Smear Microscopy Tuberculosis: a systematic review" Lancet Infectious Diseases 6(10): 664-674, 2006
4. Steingart , K. . "Sputum Processing Methods to Improve the Sensitivity of Smear Microscopy Tuberculosis: a systematic review" Lancet Infectious Diseases 6(10): 664-674, 2006
5. Shankar, R. . "Sputum Grading as a Predictor of Treatment Outcome of New Sputum Smear Positive Tuberculosis Patients in Khammam Tuberculosis Unit" National Journal of Research in Community Medicine 2(2): 116, 2013
6. Shankar, R. . "Sputum Grading as a Predictor of Treatment Outcome of New Sputum Smear Positive Tuberculosis Patients in Khammam Tuberculosis Unit" National Journal of Research in Community Medicine 2(2): 116, 2013
7. Raipal, S. , Dhingra, V. K., Aggarwal, J. K.. "Sputum Grading as Predictor of Treatment Outcome in Pulmonary Tuberculosis" Indian Journal of Tuberculosis 49, 139-141, 2002
8. Raipal, S. , Dhingra, V. K., Aggarwal, J. K.. "Sputum Grading as Predictor of Treatment Outcome in Pulmonary Tuberculosis" Indian Journal of Tuberculosis 49, 139-141, 2002
9. Van Rie, A. . "Sputum Smear Microscopy: Evaluation of Impact of Training, Microscope Distribution, and Use of External Quality Assessment Guideline for Resource-Poor Settings " Journal of Clinical Microbiology 46(3): 897-901, 2008
10. Van Rie, A. . "Sputum Smear Microscopy: Evaluation of Impact of Training, Microscope Distribution, and Use of External Quality Assessment Guideline for Resource-Poor Settings " Journal of Clinical Microbiology 46(3): 897-901, 2008

Copyright © One Window Project 2018. All rights reserved.