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Submitted: 28 December 2017 Modified: 08 August 2018
HERDIN Record #: R07-CIM-17122716495447

A study on the anti-fungal property of Capsicum frutescens (Chilli pepper) leaves against Candida albicans.

Dominique Autentico,
Emilio Jaybee Borgonia,
Mar Verdel Figuracion,
Stephanie  Hernandez,
Zandra Jarito,
Hyacith Jan Lasco,
Cristyne Loquero,
Prince Dane Manulat,
Abdulahi Mohammed,
Dominique Therese Palma,
Norman Rex Pastoriza,
ramon pono,
Alex Sanchez,
Kristine Alessandra Sy,
Noelle  Tansingco,
Matt Kristoffer  Tipgos

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Context/Background: Candida albicans is part of the normal flora of humans but may cause a disease called Candidiasis in immunocompromised individuals. The Capsicum frutescens plant is common here in the Philippines and its fruit has been proven to have many antifungal properties. However, there have been no proven studies done on the antifungal properties of its leaf extract against Candida albicans. Objective: To compare the antifungal activity of Capsicum frutescens leaf extract against Candida albicans using ethanol and chloroform as both solvents and negative control and Fluconazole as the positive control. Study Design: This study is of an experimental type of study. Study Setting: This study was conducted in Cebu Institute of Medicine Microbiology Laboratory, in F. Ramos St., Cebu City. Study Sample: The computed sample size for this study was 40 discs per solvent. This approximates to 7 plates per group. Maneuver: The method used for antifungal susceptibility testing was the Kirby-Bauer disc diffusion method using an MHIA agar plate containing 2% glucose and methylene blue. The zones of inhibition for each disc was measured and recorded. The data was then organized and analyzed. Results and Discussion: In the study, positive control showed the highest zone of inhibition among the three groups which was consistent with the pharmacological effect of the drug. Inferential statistics were not applied because based on the descriptive statistics shown above the mean zones of inhibition produced using ethanol as the solvent and using chloroform as the solvent were 8.021 and 9.690 respectively. These results are considered inactive (<10mm) based on the criteria for interpretation. Conclusion: Therefore the 100% leaf extract of Capsicum frutescens has no antifungal property against Candida albicans.


 

Publication Type
Thesis/Dissertations
Thesis Degree
MD
Specialization
Medicine
Publication Date
2014
LocationLocation CodeAvailable FormatAvailability
Cebu Institute of Medicine Abstract
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