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Submitted: 21 February 2018 Modified: 06 November 2018
HERDIN Record #: R07-CIM-18022117102793

Airborne bacterial assessment in Cebu Velez General Hospital - Intensive Care Unit.

Bianca Iris M. Anislag,
Ian Charles Axl L. Cataluña,
Sean Elvin C. Cimafranca,
Katrina Isabel T. Dilao,
Hainey Gayle Q. Galleros,
Megan R. Go,
Jessa Rose C. Inigo,
Krissa Mae  D. Lapiz,
Franzis Mari G. Lawas,
Edgar S. Llera Jr.,
Camille Isabella B. Muynila,
Shiela Cres L. Nicolas,
Shayne S. Permale,
Keth Noren D. Reston,
Edmund Jay  Y. Sy

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Context/Background: Hospital-acquired infection is a critically increasing problem in the ICU, especially because of conditions that make the patients susceptible to infection. In addition, being in an enclosed space with poor indoor air quality may place the patients at greater risks of acquiring infections. This study aimed to assess the air quality of the CVGH-ICU as poor air quality can have implications on the department maintenance protocol and patient care. Study Objectives: To assess the air for airborne bacteria in Cebu Velez General Hospital- Intensive care unit (CVGH-ICU) by identifying the three most prevalent bacteria collected from the site and by determining the air quality of CVGH-ICU based on the Polish norm for air purity protection. Methodology: Settle plates containing nutrient agar were placed on 1 stand 1 meter in height, were distributed in designated areas in the ICU and were exposed for one hour. The plates were then incubated at 37 0C for 24 hours. The top three organisms were identified using colonial morphologic characteristics, microscopic morphology, and biochemical identification tests. Bacterial load was calculated using Omelianski's formula for the settle-plate method and was expressed in CFU/m3. The total averaged bacterial load in each sampling station were evaluated according to the Polish Norm for Air Purity Protection. Results and Analysis: The three most prevalent bacteria based on bacterial load are Staphylococcus aureus, Bacillus sp., and gram-negative rods with bacterial loads of 58.81CFU/m3, 37.64 CFU/m3, and 14.91 CFU/M3, respectively. The CVGH-ICU had an average bacterial load of 58.52 CFU/m3 which falls under 'No Pollution' range of the Polish norm for Air Purity Protection. Conclusions: The three most prevalent bacteria of the CVGH-ICU based on bacterial load are Staphylococcus aureus, Bacillus sp., and gram-negative rods. Based on the Polish norm for Air Purity Protection, there is no air pollution in CVGH-ICU. Recommendations: The researchers recommend: additional biochemical testing to further the identification of the isolated microorganisms, sampling to be done in successive and consistent intervals, and expanding the scope to include microorganisms other than bacteria.

Publication Type
Thesis Degree
Publication Date
April 2016
LocationLocation CodeAvailable FormatAvailability
Cebu Institute of Medicine Abstract Print Format
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