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Submitted: 24 September 2018 Modified: 24 September 2018
HERDIN Record #: R07-USC-18092410483444

Acute and chronic toxicity studies of Lagnob (Ficus septica Burm. F. 1768) fruit extract on albino rats (Rattus norvegicus).

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Lagnob (Ficus septica) contained medicinal principles and it showed various pharmacological activities such as analgesic, antifungal, diuretic and laxative but no toxicity studies have been considered yet. The aims of this study are to determine the acute toxicity of the test solution as manifested by behavioral changes and mortality, to determine LD50 of the test solution, and to determine chronic toxicity of the test solutions as indicated through behavioral changes and vital organ weights. The test solutions where prepared by maceration using ethanol and decoction prepared into three different doses, 100mg/KBW, 500mg/KBW and 1000mg/KBW. In the study five rats were used for each dose. The test solutions were administered to the rats orally in both tests. In 24 hours acute toxicity study lasted for 45 days administration of both extracts. The rats excreted more stools in a day than normal and a presence of rectal bleeding, mortality and changes in vital organ weights were recorded and white fat-like in stool was observed. The ethanol free extract exhibit lesser effect compared to the aqueous free extract. The acute toxicity study indicates that the LD50 of the fruit extract Ficus septica is more than the limit dose and is relatively safe when administered orally in rats, but not safe when use chronically.

Publication Type
Thesis/Dissertations
Thesis Degree
BS
Specialization
Pharmacy
Publication Date
March 2015
LocationLocation CodeAvailable FormatAvailability
University of San Carlos-Josef Baumgartner Learning Resource Center, Science and Technology Section T / J254au Fulltext Print Format (Ask the Information Officer)
1. Johnson, M . "Laboratory Mice and Rats Mater Method" 2, 113,
2. Kacew, S , Festing, M . "Role of Rat Strain in the Differential Sensitivity to Pharmaceutical Agents and Naturally Occurring Substances" J. Toxicol 47, 1-30, 2004
3. Dunnick, J , Nyasha, A . "The Toxicity and Pathology of Selected Dietary Herbal Medicines" ToxicolPathol 41, 374,
4. Nugroho, A , Hermawan, A , Nastiti, K , Suven, Elisa P, Hadibarata, T , Meiyanto, E . "Immunomodulatory Effects of Hexane Insoluble Fraction of Ficusseptica Burm. F. in Doxorubicin-treated Rats" Asian Pacific Journal of Cancer Prevention 13, 2012
5. Vital, P , Velasco, Jr., R , Demigillo, J , Rivera, W . "Antimicrobial activity, cytotoxicity and phytochemical screening of Ficusseptica Burm and Sterculiafoetida L. leaf extracts" Journal of Medicinal Plants Research 4(1): 58-63, 2010
6. Damu, A , Kuo, P , Shi, L , Li, C , Kuoh, C , Wu, P , Wu , T . "Phenanthroindolizidine Alkaloids from the Stems of Ficusseptica " 68, 1071-1075, 2005
7. Sirisha, N , Sreenivasulu, M , Sangeeta, K , Madhusudhana, C . "Antioxidant Properties Ficus Species - A Review" International Journal of PharmTech Research 2(4): 2174-2182, 2010
8. Bulus, T , Atawodi, S , Mamman, M . "Acute Toxicity effect of aqueous extract of Terminaliaavicennioides on white Albino Rats" Science World Journal 6(2): 2011
9. Diallo, A , Gadegkeku, K , Agbonon, A , Aklikokou, K , Creppy, E , Gbeassor, M . "Acute and Sub-chronic (28-day) Oral Toxicity Studies of Hydroalcohol Leaf Extract of Ageratum conyzoids L. (Asteraceae)" Tropical Journal of Pharmaceutical Research 9(5): 463-467, October 2010
10. Patel, D , Desai, S , Devkar, R , Ramachandran, A . "Acute an Subchronic toxicological Evaluation of hydro-methanolic extraction of CORADIUM SATIVUM seeds" EXCLI Journal 11, 566-575, 2012
11. Ping, K , Darah, I , Chen, Y , Sreeramanan, S , Sasidharan, S . "Acute and Subchronic Toxicity Study of Euphorbia hirta L. Methanol Extract in Rats" 2013(2013): 2013
12. Usman, H , Abdulrahman, F I, Usman, A . "Qualitative Phytochemical Screening and In Vitro Antimicroial Effects of Methanol Stem Bark Extract of FicusThonningii (Moraceae)" Afr J Tradit Complement Altern Med 6(3): 289-295, 2009
13. Hosseinzadeh, H , Shakib, S , Taghiabadi, E . "Acute and Subacute Toxicity of Safranal, a Constituent of Saffron, in Mice and Rats" J Pharm Res 12(1): 93-94, 2013
14. Kaneria, M , Kanani, B , Chanda, S . "Assessment of effect of hydroalcoholic and decoction methods on extraction of antioxidants from selected Indian medicinal plants" Asian Pacific J trop Biomed 2(3): 195-202, March 2012
15. Turner, O , Brabb, T , Vasbinder, M A. "Administration of Substances to Laboratory Animals: Routes of Administration and Factors to Consider" J Am Assoc lab Anim Sci 50(5): 600-613, September 2011
16. Ferdous, N , Hiridi, S U, Hannan, J . "Antinociceptive and Hypoglycemic Activities of Scindapsusofficinalis (Roxb.) Schott in Laboratory Animals" European Journal of Medicinal Plants 3(4): 561-576, 2013
17. Mutharaman, A , Singh, A . "Acute and sub-acute oral toxicity profile of Acorus calamus (Sweet flag) in rodents" Asian Pacific Journal of Tropical Biomedicine (2012): S1017-S102, 2012
18. Tarkang, P , Agbor, G , Armelle, T . "Acute and Chronic Toxicity Studies of the aqueous and ethanol leaf extract of Carica papaya Linn in Wistar rats" J. Nat. Prod. Plant Resour 2(5): 617-627, 2012
19. Walum, E . "Acute Oral Toxicity Cell and Molecular Biology, Research Department, Pharmacia & Upjohn, Stockholm, Sweden." Environmental Health Perspectives 106, April 2004
20. "Acute toxicity testing of chemicals:opportunities to avoid redundant testing and use alternative approaches" New Centre for the Replacement, Refinement and Reduction of Animals in Research , 2004
21. Builders, M I, Isichie, C O, Aguiyi, J C. "Toxicity Studies of the Extracts of Parkia biglobosa Stem Bark in Rats" British Journal of Pharmaceutical Research 2(1): 1-16, 2012
22. Yano, Michael B, Sellers, R , Perry, R , Norton, D , Johnson, J K. "Evaluation of Organs Weights for Rodent and No-rodent Toxicity Studies: A review of Regulatory Guidelines and a survey of Current Practices" 35(5): 2007
23. Committee on Pestici, . Pesticides in the Diets of Infants and Children 2004. 146.
24. Klaassen, C . Casarett and Doull 2008. 235-236.
25. Handa, S S, Khanuja, S P, Longo, G , Rakesh, D V. Extraction technologies for medicinal and aromatic plants, International centre for science and high technology 2008.
26. National Health and Medical Research Council; Commonwealth Scientific and Industrial Research Organisation: Australian Research Council; Australian Vice-Chancellors 2004.
27. Aguinaldo, , Espeso, B , Guevara, B , Nonato, M . A Guidebook to Plant Screening: Phytochemical and Biological Reverse edition 2005.
28. Barrueto, Jr., F . (2013, November 30). Herb poisoning. . Retrieved from: http://emedicine.medscape.com/article/817427-overview#aw2aab6b2b2
29. (2010, November 30). Fucusseptica Burm. L.. Wikiherb.info. . Retrieved from: http://www.wikiherb.info/2011/01/ficus-septica-burml.html
30. Pari, . (2009, November 30). Hauli (Ficus septic Burm. F. var. septicaMoraceae). . Retrieved from: http://pari-parisblog.blogspot.com/2010/03/hauli-ficus-septic-burm
31. Abbas, . (2011, November 30). Tropical Fig tree, Ficusseptica:Health Benefits and uses of Awar-awar or Hauli. . Retrieved from: http://herbs-treatandtaste.blogspot.com/2012/03/tropical-fig-tree-ficus-septica-health.html
32. Sudarsono, . (2010, November 30). The Advantage of Medical Plant Awar-Awar (Ficusseptica). . Retrieved from: http://titan-medicalplant.blogspot.com/2011/11/advantage-of-medical-plant-awar.html
33. Saudara, . (2011, November 30). AwarAwar (FicussepticaBurm. L.). . Retrieved from: http://herbalmedicine88.blogspot.com/2012/11/awar-awar-ficus-septica-burml.html
35. Roese, J . (). The Laboratory Rat. LSSU-Institutional Animal Care & Use Committee. . Retrieved from: http://www.lssu.edu/faculty/jroese/AnimalCare/laboratory%20rats.htm
36. Ramos, J . (2006, November 30). Laxative for weight loss. . Retrieved from: http://www.fitday.com/fitness-articles/fitness/weight-loss/taking-laxatives-to-lose-weight-9-possible-consequences.html#b
37. Reyes, S . (). Eating disorder. . Retrieved from: https://www.nationaleatingdisorders.org/laxative-abuse-some-basic-facts
38. Nogodula, Judee N, Gran, Preziel F, Salamanca, Lyn Venice I., Yeo, Ellaine Anne S. Pre Clinical Evaluation of Lagnub (Ficusseptica, Moraceae) Leaf Crude Extract. 2012. University of the Immaculate Conception,

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