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Submitted: 24 September 2018 Modified: 27 September 2018
HERDIN Record #: R07-USC-18092414341269

Genotoxicity and anti-mitotic activity of "Lato", Caulerpa lentillifera (J. Agardh) Weber-van Bosse, 1898 and "Gulaman", Gracilaria verrucosa (Hudson) Papenfuss 1950.

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Algae are said to be one of the most promising long-term sustainable sources of food, feed and other products.  Algae have been used in animal and human diets since very early times. In the study, the researchers determined the genotoxicity using Allium cepa assay and anti-mitotic activity using chromosome aberration assay of Caulerpa lentillifera (lato) and Gracilaria verrucosa (gulaman). The extracts from decoction, Dichloromethane, N-hexane and Ethanol with 100% and 50% concentration test solution were tested on root meristems of Allium cepa and on sea urchin zygotes. The result showed that both 100% and 50% of the different test solution exhibit inhibition. The abnormalities observed macroscopically in the root meristems of Allium cepa were stunted growth, wilt and discoloration and microscopically were inhibition of the cells, stopping at interphase stage. The abnormalities observed in the sea urchins zygotes were cell busting, cell morphological distortion and cell inhibition. The result of this study confirmed that the decocted extracts, extracts of dichloromethane, N-haxane and ethanolic extracts of lato and gulaman exerted genotoxic and anit-mitotic effect. The constituents that may be responsible for its effects are for lato (Caulerpa lentillifera) there is the presence of alkaloid which is the caulerpin and  caulerpicin and for the gulaman (Gracilaria verrucosa) there is the  presence of eicosapentaenoic acids which are precursors of prostaglandins.

Publication Type
Thesis Degree
Publication Date
March 2015
LocationLocation CodeAvailable FormatAvailability
University of San Carlos-Josef Baumgartner Learning Resource Center, Science and Technology Section T / D336j Fulltext Print Format (Ask the Information Officer)
1. Sousa, L , et. al., . "Anti-mitotic activity towards sea urchin effs of dichloromethane fraction obtained from Allamanda schotti Pohl (Apocynaceae)" Revista Brasileira de Farmacognosia Brazilian Journal of Pharmacognosy 19(2A): 349-352, 2009
2. Ping, Kwang Yuet , et. al., . "Cytotoxicity and genotoxicity assessment of Euphorbia hirta in MCF-7 cell line model using comet assay" Asian Pacific Journal of Tropical Biomedicine 3(9): 692-696, 2013
3. Firbas, P , Tomas, A . "Allium Chromosome Aberration Test for Evaluation Effect of Cleaning Municipal Water with Constructed Wetland (CW) in Sveti Tomaz, Slovania Firbas and Amon" J Biomed Biodeg 4(4): 2013
4. Belleza, D , Liao, L . "Taxonomic Inventory of the Marine Green Algal Genus Caulerpa (Chlorohyta, Bryopsidales) at the University of San Carlos (Cebu) HERBARIUM" Philipp. Scient. 44, 71-104, 2007
5. Ghangale, G D, et. al., . "Antimitotic Activity of Lantana Camara Flowers" International Journal of Institutional Pharmacy and Life Sciences 1(1): 2011
6. Trivedil, A K, Ahmad, I . "Genotoxicity of chrysotile asbestos on Allium cepa L. meristematic root tip cells India Current Science" 105(6): 25, 2013
7. MacArtain , P , et. al., . "Nutritional Value of Edible Seaweeds" Special Article December (I): 535-543, December 2007
8. Iyer, R , et. al., . "Morphological and taxonomic studies of Gracilaria and Gracilariopsis species (Gracilariales, Rhodophyta)" South African Journal of Botany 70(4): 521-539, 2004
9. Thenmozhil, A , Mahadeva, Rao . "Evaluation of Anitmitotic Activity of Solanum Torvum Using Allium Cepa Root Meristamatic Cells and Anticancer Activity Using MCF-7-Human Mammary Gland Breast Adenocarcinoma Cell Lines" Drug Invention Today 3(12): 290-296, 2011
10. Abolghasem, J , Hamed, P . (2011, November 30). Genotoxic Impurities in Pharmaceuticals, Toxicity and Drug. . Retrieved from:
11. (). Culerpa and its Cultivation. . Retrieved from:
12. De Almeida, C , et. al., . (2010, November 30). Bioactivities from Marine Algae of the Genus Gracilaria. . Retrieved from:
13. Fernande, Dolores DG . (2004, November 30). What you really get from . . Retrieved from:
14. (). Encyclopedia Britannica. . Retrieved from:
15. Firbas, P , Tomaz, A . (2012, November 30). Allium Chromosome Aberration Test for Evaluation Effect of Cleaning Municipal Water with Constructed Wetland (CW) in Sveti Tomaz, Slovenia. . Retrieved from:
16. Ping, Kwan Yuet , et. al., . (2011, November 30). Genotoxicity of Euphorbia hirta: An Allium cepa Assay. . Retrieved from:
17. Nagappan, T , Vairappan, C . (2012, November 30). Nutritional and bioactive properties of three edible species of green algae, genus Caulerpa (Caulerpaceae). . Retrieved from:
18. Ilustrisimo, C , et. al., . Growth Performance of Caulerpa lentillifera (LATO) in Lowered Seawater pH . 2013. Philippine Science High School-Central Visayas Campus,
19. Suaring, C , Yana, A . Genotoxicity of the Fresh Mature Leaves of Mangrove Apple, Sonneratia alba Linne. 2011. University of San Carlos,
20. See, G , Lopez, E . Cytotoxic effect of Fern Frond Extract, Diplazium esculentum retz on the Embryonic Development of edible Sea Urchin, Tripnuestes gratilla Linne. 2010. University of San Carlos,

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