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Submitted: 12 November 2018 Modified: 10 January 2019
HERDIN Record #: R07-CIM-18111210334525

Effect of selected orange-flavored powdered juices on the mitotic activity of Onion (Allium cepa) root tips.

Karen Anne M. Adolfo,
Kim Harold G. Angtud,
Daphne Gay M. Cadorna,
Auggie G. Gachalian,
Leslie Ryan T. Go,
Amber U. Lee,
Karina Angela G. Lipardo,
Deborah C. Meribole,
Rachel Anne O. Monteclaro,
Lourenz A. Peralta,
Cypress Dianne P. Regis,
Xenia Marsha E. Vergara,
David Angelo A. Villacarlos,
Kimberly Ann B. Villaflor

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Low cost and palatability encourage many to drink powdered orange juices. It is consumed by many to alleviate thirst during hot seasons especially in a tropical country like the Philippines. These juices have  high content of ascorbic acid which is proven to have beneficial effects in the body.  Vitamin C, however, is only one of the myriad substances present in these processed products. Information on the effects of the other ingredients is scarce; therefore, this study was designed to determine the cumulative genotoxic effects of all ingredients present in these juices. The objectives were to determine whether or not artificially-flavored orange powdered juices have mutagenic effects on the mitotic activity of onion root tips by using the Allium cepa test and to determine if there was a significant difference between three different leading brands of powdered juices. Results showed that all three brands tested had significantly lower total number of dividing cells and hence had significantly (p<0.05) lower Mitotic Index as compared to the negative control group. This may be accounted for by the presence of well studies mutagenic substances such as tartrazine, aspartame, acesulframe-potassium, flavonoids and phenolic compounds found in all of the juices tested. Brand A was the only brand found to have an average mitotic index (7.29%) that is not significantly different from that of the positive control (8.92%), which is a herbicide shown to have genocidal effects on animal and plant studies. The two ingredients present in brand A but not in brand B and C, Sodium carboxymethyl cellulose and an unnamed iron salt, have not yet been proven to be cytotoxic. It was, therefore, concluded that powdered orange juices can significantly affect mitotic activity but whether such result was caused by a particular ingredient or differences in the amount these substances was beyond the scope of this study and were not determined. It was therefore recommended that further studies be conducted to determine the genotoxic effects of each of the specific compound found in the powdered orange juices.

Publication Type
Thesis/Dissertations
Thesis Degree
MD
Specialization
Medicine
Publication Date
2016
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